Anti-DCDC2 antibody (ab45868)

Western blot - DCDC2 antibody (ab45868)

All lanes : Anti-DCDC2 antibody (ab45868) at 0.2 µg/ml

Lane 1 : COS7 cell lysate transfected with full length recombinant human DCDC2
Lane 2 : Untransfected control COS7 cells


Predicted band size : 53 kDa
Observed band size : 53 kDa


Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot - DCDC2 antibody (ab45868)

All lanes : Anti-DCDC2 antibody (ab45868) at 1/500 dilution

Lane 1 : adult mouse heart
Lane 2 : adult mouse brain
Lane 3 : three-month old mouse brain
Lane 4 : 5 week old mouse brain
Lane 5 : mouse hippcampus including dentate gyrus
Lane 6 : mouse DRG
Lane 7 : primary mouse neuronal culture
Lane 8 : mouse astrocyte culture

Lysates/proteins at 20 µg per lane.

Secondary
DAKO cytomation Conjugation: HRP
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 15 kDa (possible non-specific binding),37 kDa (possible non-specific binding).

Exposure time : 1 minute

Lane 1: adult mouse heart was run as a negative control Lanes 6, 7 & 8 were run as positive controls

Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-DCDC2 antibody (ab45868)

ab45868 staining rat optic nerve sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected and sectioned using a cryostat. Staining with ab45868 at a 1/200 dilution in 0.3% TritonX with 0.1x PBS in 10% donkey serum was performed for 1h at 24°C. A donkey anti-goat Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody. β III tubulin can be observed in red (Alexa fluro 568 secondary). DCDC2 is expressed in the optic nerve.

This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury