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Western blot - DCDC2 antibody (ab45868)
Western blot - DCDC2 antibody (ab45868)
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Western blot - DCDC2 antibody (ab45868)
Western blot - DCDC2 antibody (ab45868)
(enlarge)


Product Name 

DCDC2 antibody

 

See all DCDC2 antibodies (2)...

Product type 

Primary antibodies

Description 

Goat polyclonal to DCDC2

Immunogen 

Synthetic peptide: C-DGEKANKDA EQKED, corresponding to Internal sequence amino acids 352-365 of Human DCDC2
BLAST 'C-DGEKANKDAEQKED' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Dog
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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• WB

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WB
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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:4,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

COS7 cell lysate.

Research areas 

Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin

Relevance 

The DCDC2 protein has two doublecortin peptide domains. This domain has been demonstrated to bind tubulin and enhance microtubule polymerization. Mutations in the gene encoding DCDC2 have been associated with Reading Disability (RD), also referred to as developmental dyslexia.

 

DCDC2 antibody (ab45868)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51473    (Human)
195208    (Mouse)

Omim

    

605755    (Human)

SwissProt

    

Q9UHG0    (Human)
Q5DU00    (Mouse)

Unigene

    

61345    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DCDC2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for DCDC2 antibody images, references, products related to ab45868 and other tools.

 

DCDC2 antibody images:

Product image  Western blot - DCDC2 antibody (ab45868)

Western blot - DCDC2 antibody (ab45868)

All lanes : DCDC2 antibody (ab45868) at 0.2 µg/ml

Lane 1 : COS7 cell lysate transfected with full length recombinant human DCDC2
Lane 2 : Untransfected control COS7 cells


Predicted band size : 53 kDa
Observed band size : 53 kDa


Primary incubation was 1 hour. Detected by chemiluminescence.

 

Product image  Western blot - DCDC2 antibody (ab45868)

Western blot - DCDC2 antibody (ab45868)

All lanes : DCDC2 antibody (ab45868) at 1/500 dilution

Lane 1 : adult mouse heart
Lane 2 : adult mouse brain
Lane 3 : three-month old mouse brain
Lane 4 : 5 week old mouse brain
Lane 5 : mouse hippcampus including dentate gyrus
Lane 6 : mouse DRG
Lane 7 : primary mouse neuronal culture
Lane 8 : mouse astrocyte culture

Lysates/proteins at 20 µg per lane.

Secondary
DAKO cytomation Conjugation: HRP
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 15 kDa (possible non-specific binding),37 kDa (possible non-specific binding).

Exposure time : 1 minute

Lane 1: adult mouse heart was run as a negative control Lanes 6, 7 & 8 were run as positive controls

Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

See Abreview

 
 

Search PubMed (MEDLINE) for references to DCDC2

 

DCDC2 antibody - more information

Customer reviews (feedback) regarding DCDC2 antibody

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DCDC2 antibody - related products:

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Lysates

 

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