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Anti-DCDC2 antibody (ab45868)

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Overview

Product name

Anti-DCDC2 antibody
See all DCDC2 products (4) ...

Description

Goat polyclonal to DCDC2

Tested applications

ELISA, WB, IHC-FoFrmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Dog

Immunogen

Synthetic peptide: C-DGEKANKDA EQKED, corresponding to Internal sequence amino acids 352-365 of Human DCDC2

C-DGEKANKD AEQKED

Positive control

COS7 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - DCDC2 antibody (ab45868)Western blot - DCDC2 antibody (ab45868) image (enlarge)

  • Western blot - DCDC2 antibody (ab45868)Western blot - DCDC2 antibody (ab45868) image (enlarge)

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-DCDC2 antibody (ab45868)Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-DCDC2 antibody (ab45868) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab45868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

May be involved in neuronal migration during development of the cerebral neocortex.

Tissue specificity

Ubiquitously expressed. In brain, highly expressed in the entorhinal cortex, inferior temporal cortex, medial temporal cortex, hypothalamus, amygdala and hippocampus.

Involvement in disease

Defects in DCDC2 may be a cause of susceptibility to dyslexia type 2 (DYX2) [MIM:600202]; also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability.

Sequence similarities

Contains 2 doublecortin domains.

Target information above from: UniProt accessionQ9UHG0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DCDC 2 antibody
  • DCDC2 antibody
  • DCDC2_HUMAN antibody
  • DCDC2A antibody
  • Doublecortin domain containing 2 antibody
  • Doublecortin domain-containing protein 2 antibody
  • HGNC:18141 antibody
  • KIAA1154 antibody
  • Protein RU2S antibody
  • RU2 antibody
  • RU2S antibody
  • RU2S protein antibody
see all

Anti-DCDC2 antibody images:

  Western blot - DCDC2 antibody (ab45868)

Western blot - DCDC2 antibody (ab45868)

All lanes : Anti-DCDC2 antibody (ab45868) at 0.2 µg/ml

Lane 1 : COS7 cell lysate transfected with full length recombinant human DCDC2
Lane 2 : Untransfected control COS7 cells


Predicted band size : 53 kDa
Observed band size : 53 kDa


Primary incubation was 1 hour. Detected by chemiluminescence.

  Western blot - DCDC2 antibody (ab45868)

Western blot - DCDC2 antibody (ab45868)

All lanes : Anti-DCDC2 antibody (ab45868) at 1/500 dilution

Lane 1 : adult mouse heart
Lane 2 : adult mouse brain
Lane 3 : three-month old mouse brain
Lane 4 : 5 week old mouse brain
Lane 5 : mouse hippcampus including dentate gyrus
Lane 6 : mouse DRG
Lane 7 : primary mouse neuronal culture
Lane 8 : mouse astrocyte culture

Lysates/proteins at 20 µg per lane.

Secondary
DAKO cytomation Conjugation: HRP
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 15 kDa (possible non-specific binding),37 kDa (possible non-specific binding).

Exposure time : 1 minute

Lane 1: adult mouse heart was run as a negative control Lanes 6, 7 & 8 were run as positive controls

Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

See Abreview

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-DCDC2 antibody (ab45868)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-DCDC2 antibody (ab45868)

ab45868 staining rat optic nerve sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected and sectioned using a cryostat. Staining with ab45868 at a 1/200 dilution in 0.3% TritonX with 0.1x PBS in 10% donkey serum was performed for 1h at 24°C. A donkey anti-goat Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody. β III tubulin can be observed in red (Alexa fluro 568 secondary). DCDC2 is expressed in the optic nerve.

This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury

See Abreview

References for Anti-DCDC2 antibody (ab45868)

ab45868 has not yet been referenced specifically in any publications.

Publishing research using ab45868? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"