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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Overview
Product name
Anti-DCDC2 antibody
See all DCDC2 products (4) ...
Description
Goat polyclonal to DCDC2
Tested applications
ELISA, WB, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Dog
Immunogen
Synthetic peptide: C-DGEKANKDA EQKED, corresponding to Internal sequence amino acids 352-365 of Human DCDC2
C-DGEKANKD AEQKED
Positive control
COS7 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-FoFr: 1/200
Target
Function
May be involved in neuronal migration during development of the cerebral neocortex.
Tissue specificity
Ubiquitously expressed. In brain, highly expressed in the entorhinal cortex, inferior temporal cortex, medial temporal cortex, hypothalamus, amygdala and hippocampus.
Involvement in disease
Defects in DCDC2 may be a cause of susceptibility to dyslexia type 2 (DYX2) [MIM:600202]; also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability.
Sequence similarities
Contains 2 doublecortin domains.
Target information above from: UniProt accessionQ9UHG0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DCDC 2 antibody
- DCDC2 antibody
- DCDC2_HUMAN antibody
see all
Database links
- Entrez Gene: 51473 Human
- Entrez Gene: 195208 Mouse
- Entrez Gene: 291130 Rat
- Omim: 605755 Human
- SwissProt: Q9UHG0 Human
- SwissProt: Q5DU00 Mouse
- SwissProt: D3ZR10 Rat
- Unigene: 61345 Human
see all
Anti-DCDC2 antibody images:
Western blot - DCDC2 antibody (ab45868)
All lanes : Anti-DCDC2 antibody (ab45868) at 0.2 µg/ml
Lane 1 : COS7 cell lysate transfected with full length recombinant human DCDC2
Lane 2 : Untransfected control COS7 cells
Predicted band size : 53 kDa
Observed band size : 53 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot - DCDC2 antibody (ab45868)
All lanes : Anti-DCDC2 antibody (ab45868) at 1/500 dilution
Lane 1 : adult mouse heart
Lane 2 : adult mouse brain
Lane 3 : three-month old mouse brain
Lane 4 : 5 week old mouse brain
Lane 5 : mouse hippcampus including dentate gyrus
Lane 6 : mouse DRG
Lane 7 : primary mouse neuronal culture
Lane 8 : mouse astrocyte culture
Lysates/proteins at 20 µg per lane.
Secondary
DAKO cytomation
Conjugation: HRP
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 15 kDa (possible non-specific binding),37 kDa (possible non-specific binding).
Exposure time : 1 minute
Lane 1: adult mouse heart was run as a negative control
Lanes 6, 7 & 8 were run as positive controls
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-DCDC2 antibody (ab45868)
ab45868 staining rat optic nerve sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected and sectioned using a cryostat. Staining with ab45868 at a 1/200 dilution in 0.3% TritonX with 0.1x PBS in 10% donkey serum was performed for 1h at 24°C. A donkey anti-goat Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody. β III tubulin can be observed in red (Alexa fluro 568 secondary). DCDC2 is expressed in the optic nerve.
This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury
References for Anti-DCDC2 antibody (ab45868)
ab45868 has not yet been referenced specifically in any publications.
Publishing research using ab45868? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"