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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Overview
Product name
Anti-DCDC2 antibody
See all DCDC2 products (4) ...
Description
Goat polyclonal to DCDC2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Human
Immunogen
Synthetic peptide: C-PQPLKRKGKKDSN, corresponding to internal sequence amino acids 271-283 of Mouse DCDC2
C-PQPLKRKG KKDSN
Positive control
Mouse Brain lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
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Western blot - DCDC2 antibody (ab53549)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Peptide ELISA: Antibody detection limit dilution 1/32000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in neuronal migration during development of the cerebral neocortex.
Tissue specificity
Ubiquitously expressed. In brain, highly expressed in the entorhinal cortex, inferior temporal cortex, medial temporal cortex, hypothalamus, amygdala and hippocampus.
Involvement in disease
Defects in DCDC2 may be a cause of susceptibility to dyslexia type 2 (DYX2) [MIM:600202]; also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability.
Sequence similarities
Contains 2 doublecortin domains.
Target information above from: UniProt accessionQ9UHG0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DCDC 2 antibody
- DCDC2 antibody
- DCDC2_HUMAN antibody
see all
Database links
- Entrez Gene: 51473 Human
- Entrez Gene: 195208 Mouse
- Entrez Gene: 291130 Rat
- Omim: 605755 Human
- SwissProt: Q9UHG0 Human
- SwissProt: Q5DU00 Mouse
- SwissProt: D3ZR10 Rat
- Unigene: 61345 Human
see all
Anti-DCDC2 antibody images:
Western blot - DCDC2 antibody (ab53549)
ab53549 antibody (0.1µg/ml) staining of DCDC2 in Mouse Brain lysate (35µg protein in RIPA buffer)
Predicted band size : 41 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-DCDC2 antibody (ab53549)
ab53549 has not yet been referenced specifically in any publications.
Publishing research using ab53549? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"