DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit (ab113851)
- Product nameDCFDA - Cellular Reactive Oxygen Species Detection Assay KitSee all Oxidative Stress kits ...
- Tests1 x 300 test
- Product overview
ab113851 contains sufficient materials for approximately 300 measurements in microplate format and 70 measurements (35 mL) by flow cytometry.
ab113851 uses the cell permeant reagent 2’,7’ –dichlorofluorescein diacetate (DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’, 7’ –dichlorofluorescin (DCF). DCF is a highly fluorescent compound which can be detected by fluorescence spectroscopy with maximum excitation and emission spectra of 495nm and 529nm respectively.
This kit is not compatible with fixed samples. Stained cells must be measured live.
Store all components at 4°C in the dark. The kits are stable for at
least 6 months from receipt. For longer term storage, keep at -20°C
to -80°C in the dark.
- Tested applicationsFlow Cyt, Functional Studies more details
Components 300 tests Dilution Buffer (10X, sterile) 1 x 10ml Label (20mM DCFDA, 1000X) 1 x 35µl TBHP (55 mM) 1 x 0.05ml
- Research Areas
Our Abpromise guarantee covers the use of ab113851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt|
DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit images
Cells labeled with ab113851 (DCFDA) (20µM) and unlabeled (none) Jurkat cells were treated with +/- 50µM tert-butyl Hydrogen Peroxide (tbHP) for 3 hours at 37°C. Cells were then washed once with PBS, transferred to a microplate and read on a spectrophotometer. Mean +/- standard deviation is plotted for 3 replicates from each condition. tbHP mimics ROS activity to oxidize DCFDA to fluorescent DCF.
ab113851 (DCFDA) labeled and unlabeled Jurkat cells were treated with 50µM tert-butyl Hydrogen Peroxide (tbHP) as described above and then analyzed by flow cytometry.
References for DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit (ab113851)
This product has been referenced in:
- Roy A & Sil PC Tertiary butyl hydroperoxide induced oxidative damage in mice erythrocytes: Protection by taurine. Pathophysiology 19:137-48 (2012). Read more (PubMed: 22626456) »