DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit (ab113851)

Overview

• Product nameDCFDA - Cellular Reactive Oxygen Species Detection Assay KitSee all Oxidative Stress kits ...
• Tests
  1 x 300 test
• Product overview

  ab113851 contains sufficient materials for approximately 300 measurements in microplate format and 70 measurements (35 mL) by flow cytometry.

   

  ab113851 uses the cell permeant reagent 2’,7’ –dichlorofluorescein diacetate (DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’, 7’ –dichlorofluorescin (DCF). DCF is a highly fluorescent compound which can be detected by fluorescence spectroscopy with maximum excitation and emission spectra of 495nm and 529nm respectively.

   

   

• Notes

  This kit is not compatible with fixed samples. Stained cells must be measured live.

   

  Store all components at 4°C in the dark. The kits are stable for at

  least 6 months from receipt. For longer term storage, keep at -20°C

  to -80°C in the dark.

• Tested applicationsFlow Cyt, Functional Studies more details

Properties

• Components	300 tests
  Dilution Buffer (10X, sterile)	1 x 10ml
  Label (20mM DCFDA, 1000X)	1 x 35µl
  TBHP (55 mM)	1 x 0.05ml

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • Cell Metabolism Kits
  • Cell Viability and Senescence Kits

  • Kits/ Lysates/ Other
  • Kits
  • Cell Damage Kits
  • Oxidative stress

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit images

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  Functional Studies - DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit (ab113851)
  Cells labeled with ab113851 (DCFDA) (20µM) and unlabeled (none) Jurkat cells were treated with +/- 50µM tert-butyl Hydrogen Peroxide (tbHP) for 3 hours at 37°C. Cells were then washed once with PBS, transferred to a microplate and read on a spectrophotometer. Mean +/- standard deviation is plotted for 3 replicates from each condition. tbHP mimics ROS activity to oxidize DCFDA to fluorescent DCF.
• 

  Flow Cytometry - DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit (ab113851)
  ab113851 (DCFDA) labeled and unlabeled Jurkat cells were treated with 50µM tert-butyl Hydrogen Peroxide (tbHP) as described above and then analyzed by flow cytometry.

Protocols

Protocol Booklet