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Product name
DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit
Tests
1 x 300 test
Product overview
ab113851 contains sufficient materials for approximately 300 measurements in microplate format and 70 measurements (35 mL) by flow cytometry.
ab113851 uses the cell permeant reagent 2’,7’ –dichlorofluorescein diacetate (DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’, 7’ –dichlorofluorescin (DCF). DCF is a highly fluorescent compound which can be detected by fluorescence spectroscopy with maximum excitation and emission spectra of 495nm and 529nm respectively.
Notes
Store all components at 4°C in the dark. The kits are stable for at
least 6 months from receipt. For longer term storage, keep at -20°C
to -80°C in the dark.
least 6 months from receipt. For longer term storage, keep at -20°C
to -80°C in the dark.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 300 tests |
|---|---|---|
| Buffer (10X) | P/N 8206005 | 1 x 10ml |
| Label (20mM DCFDA, 1000X) | P/N 8206004 | 1 x 35µl |
| TBHP (55 mM) | P/N 8206006 | 1 x 0.05ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits >> Oxidative stress kits
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Cell Viability and Senescence Kits
Cell Biology >> Other Antibodies >> Oxidative Stress
Alternative names
- MS958
Applications
Show applications key
Our Abpromise guarantee covers the use of ab113851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit images:
Functional Studies - DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit (ab113851)
Cells labeled with ab113851 (DCFDA) (20µM) and unlabeled (none) Jurkat cells were treated with +/- 50µM tert-butyl Hydrogen Peroxide (tbHP) for 3 hours at 37°C. Cells were then washed once with PBS, transferred to a microplate and read on a spectrophotometer. Mean +/- standard deviation is plotted for 3 replicates from each condition. tbHP mimics ROS activity to oxidize DCFDA to fluorescent DCF.
Flow Cytometry - DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit (ab113851)
ab113851 (DCFDA) labeled and unlabeled Jurkat cells were treated with 50µM tert-butyl Hydrogen Peroxide (tbHP) as described above and then analyzed by flow cytometry.
Protocols
References for DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit (ab113851)
ab113851 has not yet been referenced specifically in any publications.
Publishing research using ab113851? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"