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Anti-DCI antibody - C-terminal (ab93476)

Overview

  • Product nameAnti-DCI antibody - C-terminalSee all DCI primary antibodies ...
  • Description
    Rabbit polyclonal to DCI - C-terminal
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide selected from the C terminal region of Human DCI conjugated to KLH (NP_001910.2).

  • Positive controlMCF7 cell line lysate and Mouse heart tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab93476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
  • Application notesELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 33 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDCI is a member of the hydratase/isomerase superfamily and is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described, but their full length nature has not been determined.
    • Cellular localizationMitochondrial matrix
    • Database links
    • Alternative names
        3,2 trans enoyl Coenzyme A isomerase antibodyAcetylene allene isomerase antibodyD3,D2 enoyl CoA isomerase antibody
        DCI protein antibodyDelta(3),Delta(2) enoyl CoA isomerase antibodyDelta3, delta2 enoyl CoA isomerase antibodyDodecenoyl CoA delta Isomerase antibodyDodecenoyl CoA isomerase antibodyDodecenoyl Coenzyme A delta isomerase antibodyDodecenoyl Coenzyme A delta isomerase (3,2 trans enoyl Coenzyme A isomerase) antibodyDodecenoyl Coenzyme A delta isomerase 3,2 trans enoyl Coenzyme A isomerase, isoform CRA a antibody
      see all

    Anti-DCI antibody - C-terminal images

    • Anti-DCI antibody - C-terminal (ab93476) at 1/100 dilution + MCF7 cell line lysates at 35 µg

      Predicted band size : 33 kDa
    • Anti-DCI antibody - C-terminal (ab93476) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

      Predicted band size : 33 kDa

    References for Anti-DCI antibody - C-terminal (ab93476)

    ab93476 has not yet been referenced specifically in any publications.

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    Application Western blot
    Sample Mouse Tissue lysate - other (brain mitochondria)
    Loading amount 20 µg
    Specification brain mitochondria
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Verified customer

    Submitted Feb 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"