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I used a new vial of ab83046 and it is not nearly as sensitive as the last vial i bought. before i used 1:2000 with a nice band and now 1:500 gives only a faint band. |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-DCK antibody (ab83046) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 31 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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