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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-DCK antibody
See all DCK products (5) ...
Description
Rabbit polyclonal to DCK
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (ATPETCLHRI YLRGRNEEQG IPLEYLEKLH YKHESWLLHR TLKTNFDYLQ) of human DCK (NP_000779).
ATPETCLHRI YLRGRNEEQG IPLEYLEKLH YKHESWLLHR TLKTNFDYLQ
Positive control
Fetal heart lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Western blot - DCK antibody (ab83046)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:62500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
Sequence similarities
Belongs to the DCK/DGK family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP27707
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
see all
Database links
- Entrez Gene: 1633 Human
- Entrez Gene: 13178 Mouse
- Entrez Gene: 79127 Rat
- Entrez Gene: 541538 Zebrafish
- Omim: 125450 Human
- SwissProt: Q3MHR2 Cow
- SwissProt: P27707 Human
- SwissProt: P43346 Mouse
see all
Anti-DCK antibody images:
Western blot - DCK antibody (ab83046)
Anti-DCK antibody (ab83046) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 31 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-DCK antibody (ab83046)
This product has been referenced in:
- Bieghs Let al. The effects of forodesine in murine and human multiple myeloma cells. Adv Hematol 2010:131895 (2010). WB; Mouse.Read more (PubMed: 20981156) »
See 1 publication for this product
Publishing research using ab83046? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"