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Overview

  • Product nameDCK protein
  • Protein descriptionRecombinant full length Human DCK (amino acids 1-260) with N terminal His tag; 296 amino acids with tag, Predicted MWt 34.6 kDa (UniProt P27707).
  • Uniprot accessionP27707
  • Molecular weight34.600kDa inclusive of tags
  • Protein length260 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab99520 was purified using conventional chromatography.
  • Mass spectrometryMALDI-TOF
  • E.C. Number2.7.1.74
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM EDTA, 1mM DTT, 0.1mM PMSF, pH 7.5
  • Concentration information loading...
  • SequenceMRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMATP PKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQL CEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQM MYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVL FFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNN QFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEY LEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKD KYESLVEKVKEFLSTL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab99520 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS MS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab151966.

    • Protein info

    • Alternative names
        DCKDCK proteinDCK_HUMAN
        Deoxycytidine kinaseEC 2.7.1EC 2.7.1.74MGC117410MGC138632OTTHUMP00000219118OTTHUMP00000219119
      see all
  • FunctionRequired for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • Sequence similaritiesBelongs to the DCK/DGK family.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P27707 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    DCK protein images

    • SDS-PAGE - DCK protein (ab99520)
      SDS-PAGE - DCK protein (ab99520)
      15% SDS-PAGE analysis of 3µg ab99520.

    References for DCK protein (ab99520)

    ab99520 has not yet been referenced specifically in any publications.

    Publishing research using ab99520 ? Please let us know so that we can cite the reference in this datasheet

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