Anti-DCL-1 antibody (ab109991)
Key features and details
- Rabbit polyclonal to DCL-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DCL-1 antibody -
Description
Rabbit polyclonal to DCL-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
A synthesized peptide derived from internal region of Human DCL-1
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Positive control
- Human lymph node tissue HT-29 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Frozen Stock (-20C). Shelf life 12 months. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 26 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 26 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9936 Human
- Entrez Gene: 66205 Mouse
- Entrez Gene: 295629 Rat
- Omim: 612246 Human
- SwissProt: Q8IX05 Human
- SwissProt: Q9DCG2 Mouse
- SwissProt: Q5FVR3 Rat
- Unigene: 130014 Human
see all -
Alternative names
- BIMLEC antibody
- C type lectin BIMLEC antibody
- C type lectin domain family 13 member A antibody
see all
Images
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Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using ab109991. The picture on the right is blocked with the synthesized peptide.
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All lanes : Anti-DCL-1 antibody (ab109991) at 1/500 dilution
Lane 1 : HT-29 cells
Lane 2 : HT-29 cells with synthesized peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109991 has not yet been referenced specifically in any publications.