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Overview

  • Product nameDCTN1 protein (Tagged-His Tag)See all DCTN1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 22-291 (270) of Human DCTN1 isoform p135 with N terminal His tag. MWt ~31 kDa; UniProt ID = Q14203-2; NCBI accession = NP_001128513.1
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 102 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notes(Amino acid sequence (Sequence determined by 5' Sequencing))
    ASTGVAGASSSLGPSGSASAGELSSS EPSTPAQTPLAAPIIPTPVLTSPGAVPP LPSPSKEEEG LRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQ EWKS KMQEQQADLQRRLKEARKEAKEALEAKERYMEEM ADTADAIEMATLDKEMAEER AESLQQEVEALKERVDEL TTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLK DA LVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQ EELSQAESTIDE
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91679 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        150 kDa dynein associated polypeptide150 kDa dynein-associated polypeptideDAP 150
        DAP-150DAP150DCTN 1DCTN1DCTN1_HUMANDP 150DP-150DP150Dynactin 1Dynactin 1 (p150 Glued (Drosophila) homolog)Dynactin 1 (p150 glued homolog Drosophila)Dynactin subunit 1Dynactin1HMN7BP135p150 gluedp150 Glued (Drosophila) homologp150 glued homologp150(GLUED) DROSOPHILA HOMOLOG OFp150-gluedp150glued
      see all
  • FunctionRequired for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
  • Tissue specificityBrain.
  • Involvement in diseaseDefects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally.
  • Sequence similaritiesBelongs to the dynactin 150 kDa subunit family.
    Contains 1 CAP-Gly domain.
  • Post-translational
    modifications    Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton.

    • Target information above from: UniProt accession Q14203 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for DCTN1 protein (Tagged-His Tag) (ab91679)

    ab91679 has not yet been referenced specifically in any publications.

    Publishing research using ab91679 ? Please let us know so that we can cite the reference in this datasheet

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