Recombinant Anti-DCTN5 antibody [EPR11253] (ab157197)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11253] to DCTN5
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DCTN5 antibody [EPR11253]
See all DCTN5 primary antibodies -
Description
Rabbit monoclonal [EPR11253] to DCTN5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human DCTN5.
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Positive control
- MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11253 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/250 - 1/500.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
IP
1/10 - 1/100. |
Target
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Sequence similarities
Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 84516 Human
- Entrez Gene: 59288 Mouse
- Entrez Gene: 308961 Rat
- Omim: 612962 Human
- SwissProt: Q9BTE1 Human
- SwissProt: Q9QZB9 Mouse
- Unigene: 435941 Human
- Unigene: 478285 Mouse
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Alternative names
- DCTN 5 antibody
- DCTN5 antibody
- DCTN5_HUMAN antibody
see all
Images
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All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 20 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution.
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Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab157197 has been referenced in 1 publication.
- Li L et al. Data-Independent Acquisition-Based Quantitative Proteomics Analysis Reveals Dynamic Network Profiles during the Macrophage Inflammatory Response. Proteomics 20:e1900203 (2020). PubMed: 31876377