Anti-DCV capsid polyprotein antibody (ab92954)

Overview

  • Product nameAnti-DCV capsid polyprotein antibody
  • Description
    Rabbit polyclonal to DCV capsid polyprotein
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Drosophila C Virus
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of Drosophila C virus DCV capsid polyprotein.

Properties

Applications

Our Abpromise guarantee covers the use of ab92954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.

Target

  • RelevanceDCV capsid polyprotein (DCVgp2) includes rhv_like or Picornavirus capsid protein domain_like and CRPV_capsid domains. Picornaviruses are non-enveloped plus-strand ssRNA animal viruses with icosahedral capsids composed of 60 copies each of 4 virus encoded proteins. Alignment includes picornaviridae, like poliovirus, hepatitis A virus, rhinovirus, foot-and-mouth disease virus and encephalomyocarditis virus. The common structure of DCV capsid polyprotein is an 8-stranded beta sandwich.
  • Database links
    • Alternative names
      • DCVgp2 antibody
      • DCVgp2 capsid polyprotein antibody

    References for Anti-DCV capsid polyprotein antibody (ab92954)

    ab92954 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 500000 cells
    Gel Running Conditions Non-reduced Denaturing (12 %)
    Sample Drosophila C Virus Cell lysate - whole cell (Drosophila S2 cell)
    Specification Drosophila S2 cell
    Treatment DCV MOI=0,1 0,01 or 0,001 for 2hrs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Ms. Barbara Viginier

    Verified customer

    Submitted Jan 22 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"