![Western blot - Anti-DCXR antibody [4G4AF5] (ab110283) image](#DCXR-Primary-antibodies-ab110283-4.gif)
![Immunocytochemistry/ Immunofluorescence - Anti-DCXR antibody [4G4AF5] (ab110283) image](#DCXR-Primary-antibodies-ab110283-5.gif)
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Product name
Anti-DCXR antibody [4G4AF5]
See all DCXR products (6) ...
Description
Mouse monoclonal [4G4AF5] to DCXR
Tested applications
WB, ICC/IF, In-Cell ELISA, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Human liver mitochondria
Positive control
Isolated human liver mitochondria; Human HDFn cells; HL-60 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. Purity near homogeneity as judged by SDS-PAGE.
Clonality
Monoclonal
Clone number
4G4AF5
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF: Use a concentration of 4 µg/ml.
In-Cell ELISA: Use a concentration of 4 µg/ml. (0.4 µg/well).
Flow Cyt: Use a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
Target
Function
Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
Tissue specificity
Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis.
Involvement in disease
Note=The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose.
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Cellular localization
Membrane. Probably recruited to membranes via an interaction with phosphatidylinositol.
Target information above from: UniProt accessionQ7Z4W1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- L xylulose reductase antibody
- Carbonyl reductase 2 antibody
- Carbonyl reductase II antibody
see all
Database links
- Entrez Gene: 51181 Human
- Omim: 608347 Human
- SwissProt: Q7Z4W1 Human
- Unigene: 9857 Human
Anti-DCXR antibody [4G4AF5] images:
Western blot - Anti-DCXR antibody [4G4AF5] (ab110283)
![Western blot - Anti-DCXR antibody [4G4AF5] (ab110283)](/ps/datasheet/images/110/ab110283/DCXR-Primary-antibodies-ab110283-4.gif)
Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µg
Secondary
Goat anti-mouse conjugated to AP
Predicted band size : 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DCXR antibody [4G4AF5] (ab110283)
![Immunocytochemistry/ Immunofluorescence - Anti-DCXR antibody [4G4AF5] (ab110283)](/ps/datasheet/images/110/ab110283/DCXR-Primary-antibodies-ab110283-5.gif)
Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.
Flow Cytometry - DCXR antibody (ab110283)
HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
References for Anti-DCXR antibody [4G4AF5] (ab110283)
ab110283 has not yet been referenced specifically in any publications.
Publishing research using ab110283? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"