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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab2231? Please let us know so that we can cite the reference in this datasheet
ab2231 has been referenced in 3 publications.
- Shao Z et al. Pulmonary hypertension associated with advanced systolic heart failure: dysregulated arginine metabolism and importance of compensatory dimethylarginine dimethylaminohydrolase-1. J Am Coll Cardiol 59:1150-8 (2012). WB; Human. PubMed: 22440215
- Ito E et al. Regulation of myometrial contractivity during pregnancy in the rat: potential role for DDAH. Mol Hum Reprod 15:507-12 (2009). PubMed: 19528264
- Hasegawa K et al. Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2. Circ Res 101:e2-10 (2007). PubMed: 17601800
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - DDAH1 antibody (ab2231)
Predicted band size : 31 kDa
ab2231 (0.1 µg/ml) staining human kidney whole tissue lysate (35 µg) in RIPA buffer. Incubation with primary antibody was for 1 hour. Detection was by chemiluminescence.
Immunocytochemistry/ Immunofluorescence - DDAH1 antibody (ab2231)
ICC/IF image of ab2231 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2231, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-DDAH1 antibody(ab2231)
IHC image of ab2231 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2231, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
DDAH1 antibody for Western blot in Mouse (2231)
DDAH1 antibody for WB in Rat (2231)
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