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Western blot - DDB2 antibody [2246C4a] (ab51017)
![Western blot - DDB2 antibody [2246C4a] (ab51017)](/ps/datasheet/images/ddb2.jpg)
(enlarge)
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Product Name
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DDB2 antibody [2246C4a]
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See all DDB2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2246C4a] to DDB2
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Immunogen
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Recombinant fragment (N-terminal) Human.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Dot, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependent dilution.
WB: 1/50. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
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Relevance
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DDB2 is required for expression of an ultraviolet radiation (UV)-damaged DNA-binding activity and is disrupted by mutations in the subset of xeroderma pigmentosum group E cells that lack this activity, DDB-negative XPE. DDB2 is the smaller subunit of a heterodimeric protein implicated in the etiology of xeroderma pigmentosum group E. This subunit appears to be required for DNA binding.
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DDB2 antibody [2246C4a] (ab51017) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2246C4a
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DDB2 antibody [2246C4a] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DDB2 antibody [2246C4a] images, references, products related to ab51017 and other tools.
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DDB2 antibody [2246C4a] images:
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 Western blot - DDB2 antibody [2246C4a] (ab51017)
DDB2 antibody [2246C4a] (ab51017) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
developed using the ECL technique
Predicted band size : 48 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to DDB2
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DDB2 antibody [2246C4a] - more information
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Customer reviews (feedback) regarding DDB2 antibody [2246C4a] |
Customer FAQs regarding DDB2 antibody [2246C4a] |
Protocols for DDB2 antibody [2246C4a] |
Price and availability of products related to DDB2 antibody [2246C4a] |
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DDB2 antibody [2246C4a] - related products:
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DDB2 antibody [2246C4a] - other tools:
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Contact Abcam with a Technical Enquiry about DDB2 antibody [2246C4a] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - DDB2 antibody [2246C4a] (ab51017)](/index.html?datasheet=51017#ddb2.jpg)
