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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
Anti-DDB2 antibody [2246C4a]
See all DDB2 products (2) ...
Mouse monoclonal [2246C4a] to DDB2
IHC-P, ICC/IF, WB, Dot Blot, Flow Cytmore details
Reacts with
Human
Recombinant fragment (N-terminal) Human.
HeLa whole cell lysate
Liquid
Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Protein G purified
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
Monoclonal
2246C4a
IgG1
Our Abpromise guarantee covers the use of ab51017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 10 µg/ml
WB: 1/50Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
Dot: Use at an assay dependent dilution.
Flow Cyt: Use 1µg for 106 cells.
DDB2 is required for expression of an ultraviolet radiation (UV)-damaged DNA-binding activity and is disrupted by mutations in the subset of xeroderma pigmentosum group E cells that lack this activity, DDB-negative XPE. DDB2 is the smaller subunit of a heterodimeric protein implicated in the etiology of xeroderma pigmentosum group E. This subunit appears to be required for DNA binding.
Nuclear
Western blot - DDB2 antibody [2246C4a] (ab51017)
![Western blot - DDB2 antibody [2246C4a] (ab51017)](/ps/datasheet/Images/51/ab51017/ddb2.jpg)
Anti-DDB2 antibody [2246C4a] (ab51017) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
developed using the ECL technique
Predicted band size : 48 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-DDB2 antibody [2246C4a](ab51017)
](/ps/datasheet/images/51/ab51017/DDB2-Primary-antibodies-ab51017-1.jpg)
IHC image of ab51017 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence-DDB2 antibody [2246C4a](ab51017)
](/ps/datasheet/images/51/ab51017/DDB2-Primary-antibodies-ab51017-3.jpg)
ICC/IF image of ab51017 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-Anti-DDB2 antibody [2246C4a](ab51017)
](/ps/datasheet/images/51/ab51017/DDB2-Primary-antibodies-ab51017-4.jpg)
Overlay histogram showing HeLa cells stained with ab51017 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
ab51017 has not yet been referenced specifically in any publications.
Publishing research using ab51017? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
![Western blot - DDB2 antibody [2246C4a] (ab51017)](/ps/datasheet/Images/51/ab51017/ddb2.jpg)
Anti-DDB2 antibody [2246C4a] (ab51017) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
developed using the ECL technique
Predicted band size : 48 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
](/ps/datasheet/images/51/ab51017/DDB2-Primary-antibodies-ab51017-1.jpg)
IHC image of ab51017 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
](/ps/datasheet/images/51/ab51017/DDB2-Primary-antibodies-ab51017-3.jpg)
ICC/IF image of ab51017 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
](/ps/datasheet/images/51/ab51017/DDB2-Primary-antibodies-ab51017-4.jpg)
Overlay histogram showing HeLa cells stained with ab51017 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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