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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
Overview
Product name
Anti-DDB2 antibody
See all DDB2 products (2) ...
Description
Rabbit polyclonal to DDB2
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human DDB2.
(Peptide available as ab87818.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: Hela; A431.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 µg/ml
Target
Relevance
DDB2 is required for expression of an ultraviolet radiation (UV)-damaged DNA-binding activity and is disrupted by mutations in the subset of xeroderma pigmentosum group E cells that lack this activity, DDB-negative XPE. DDB2 is the smaller subunit of a heterodimeric protein implicated in the etiology of xeroderma pigmentosum group E. This subunit appears to be required for DNA binding.
Cellular localization
Nuclear
Alternative names
- damage-specific DNA binding protein 2 antibody
- UV-DDB 2 antibody
Database links
- Entrez Gene: 1643 Human
- Omim: 600811 Human
- SwissProt: Q92466 Human
- Unigene: 446564 Human
Anti-DDB2 antibody images:
Western blot - DDB2 antibody (ab77765)
All lanes : Anti-DDB2 antibody (ab77765) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 40 kDa (possible isoform),62 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
DNA damage-binding protein 2 has five isoforms of varying molecular weights (SwissProt). Isoform 1 is the antibody in question's primary target and is seen at 47 kDa, the additional band present at 40 kDa is also an isomer of DNA damage-binding protein 2 (Isomer D3).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDB2 antibody (ab77765)
IHC image of DDB2 staining in Human Breast Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77765, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence - Anti-DDB2 antibody (ab77765)
ICC/IF image of ab77765 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77765, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-DDB2 antibody (ab77765)
This product has been referenced in:
- Stoyanova Tet al. p21 cooperates with DDB2 in suppression of UV-induced skin malignancies. J Biol Chem : (2011). IHC-P; Human.Read more (PubMed: 22167187) »
See 1 publication for this product
Publishing research using ab77765? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"