Overview
Product nameDDB2 peptideSee all DDB2 proteins and peptides ...
Protein descriptionSynthetic peptide derived from within residues 1 - 100 of Human DDB2.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab77765 - DDB2 antibody.
Protein length427 amino acids
Properties
Purity70 - 90% by HPLC
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferInformation available upon request.
Concentration information loading...
Research Areas
Applications
Application notesThis peptide can be used with studies using ab77765.
Protein info
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Alternative names
damage-specific DNA binding protein 2Damage-specific DNA-binding protein 2DDB p48 subunit
Ddb2DDB2_HUMANDDBbDNA damage-binding protein 2UV-damaged DNA-binding protein 2UV-DDB 2
see all
FunctionRequired for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.
Tissue specificityUbiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in DDB2 are a cause of xeroderma pigmentosum complementation group E (XP-E) [MIM:278740]; also known as xeroderma pigmentosum V (XP5). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities.
Sequence similaritiesBelongs to the WD repeat DDB2/WDR76 family.
Contains 5 WD repeats.
DomainThe DWD box is required for interaction with DDB1.
Post-translational
modificationsPhosphorylation by ABL1 negatively regulate UV-DDB activity.
Ubiquitinated by CUL4A in response to UV irradiation. Ubiquitination appears to both impair DNA-binding and promotes ubiquitin-dependent proteolysis. Degradation of DDB2 at sites of DNA damage may be a prerequisite for their recognition by XPC and subsequent repair. CUL4A-mediated degradation appears to be promoted by ABL1.
Cellular localizationNucleus. Accumulates at sites of DNA damage following UV irradiation.
References for DDB2 peptide (ab87818)
ab87818
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
