FITC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab2492)
Key features and details
- FITC Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
-
Product name
FITC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
FITC Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence). Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
-
General notes
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
The molar F/P ratio is 4.1.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Notes |
---|
Optimal working dilutions should be determined for your particular assay conditions.
Target
-
Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab2492 has been referenced in 4 publications.
- Gangoso E et al. Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion. Cell 184:2454-2470.e26 (2021). PubMed: 33857425
- Redinus K et al. An Hb-mediated circulating macrophage contributing to pulmonary vascular remodeling in sickle cell disease. JCI Insight 4:N/A (2019). PubMed: 31391342
- Inoue N et al. Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation. Nat Commun 9:1842 (2018). PubMed: 29743476
- Guo Q et al. TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma in vitro and in vivo. Oncol Lett 13:949-954 (2017). PubMed: 28356983