Overview

  • Product nameAnti-DDDDK tag antibody
    See all DDDDK tag primary antibodies
  • Description
    Goat polyclonal to DDDDK tag
  • Tested applicationsELISA, ICC, WB, ICC/IFmore details
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    xxxDDDDK

  • General notesEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab1257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/500.
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • flag antibody
    see all

Anti-DDDDK tag antibody images

  • Human microvascular endothelial cells expressing DDDDK-tagged beta-catenin following transient transfection.
    Ab1257 used at 1 µg/ml. Detected with FITC labeled rabbit anti-goat IgG (H&L) secondary antibody.

References for Anti-DDDDK tag antibody (ab1257)

This product has been referenced in:
  • Peters DM  et al. TGF-ß directs trafficking of the epithelial sodium channel ENaC which has implications for ion and fluid transport in acute lung injury. Proc Natl Acad Sci U S A 111:E374-83 (2014). Read more (PubMed: 24324142) »
  • Stimpson KM  et al. Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function. PLoS One 9:e92432 (2014). IF ; Human . Read more (PubMed: 24662969) »

See all 11 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample Human Cell (Vascular smooth muscle cells)
Specification Vascular smooth muscle cells
Permeabilization Yes - NP40
Fixative Formaldehyde
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Submitted Dec 16 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (COS7)
Specification COS7
Permeabilization Yes - triton X100
Fixative Formaldehyde
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Submitted Oct 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Kidney cancer cell)
Loading amount 20 µg
Specification Kidney cancer cell
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Nov 12 2010

Thank you for your enquiry. None of our FLAG tag antibodies have currently been tested in IHC on paraffin sections. Some have been tested on frozen sections (ab1257,ab21536) and as this work in this application this indicates that those antibodies ...

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Results such as these are commonly solved by loading less lysate (try 5 mcg/lane), diluting the primary antibody more (try 1:25,000) and diluting the secondary antibody more (try 1:20,000). The goat anti-ECS is 10 to 20 fold higher titered than the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"