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Anti-DDDDK tag antibody (ab21536)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab21536 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

2 questions for ab21536

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Question 1

Thursday 08-December-2005

I attached the protocol that I used. Actually, My VSVG antibody (catalog number:ab18612-200) that we ordered together with those two antibodiies worked when I used this protocol.

Thank you so much.

ANSWER:

  

Thank you for your enquiry. Jen is out of the office, so I am answering her correspondence.

I understand that you used a third antibody successfully in this protocol, but each antibody is different and sometimes must be optimized to work well.

What is the specific problem you are getting with each antibody? Are you seeing no signal whatsoever, high background, etc?

What blocking solution did you use? 5% milk? BSA?

How long and at what temperature did you incubate with the primary antibody? The protocol you included said 2-3 hours at room temperature or overnight at 4 degrees.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Question 2

Tuesday 28-June-2005

the spec sheet does not indicate wheter the antibody reacts with N- terminus or C- terminus FLAG - do you have the answer ? Our FLAG is fused to the N- terminus thanks - thanks for your help

ANSWER:

  

Thank you for your enquiry. This antibody should recognize both N- terminus or C- terminus DDDDK tag.

If you have any additional questions, please contact us again.

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