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Products:Tags & Cell Markers >> Epitope Tags >> DDDDK Tag
Overview
Product name
Anti-DDDDK tag antibody
See all DDDDK tag products (34) ...
Description
Rabbit polyclonal to DDDDK tag
Tested applications
IHC-P, IHC-Fr, ELISA, WB, ICCmore details
Immunogen
Synthetic peptide: XXXDDDDK conjugated to KLH. XXX represents random amino acids. This sequence represents the Enterokinase Cleavage Site
XXXDDDDK
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purification notes
ECS (Enterokinase Cleavage Site) Affinity Purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDDDK tag antibody (ab21536)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (PubMed: 20371678)
IHC-Fr: Use a concentration of 0.5 - 5 µg/ml.
ELISA: Use at an assay dependent dilution. (Use at a concentration of 2 - 10 µg/ml for coating or 0.03 - 1 µg/ml as a primary.)
WB: Use at an assay dependent dilution. (Use at a concentration of 0.1 - 1 µg/ml (colorimetric) or 0.03 - 1µg/ml (chemiluminescence).)
ICC: Use a concentration of 0.5 - 5 µg/ml.
Target
Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
Alternative names
- DDDDK epitope tag antibody
- DDDDK epitope tag antibody
- DYKDDDDK epitope tag antibody
see all
Anti-DDDDK tag antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDDDK tag antibody (ab21536)
ab21536 staining exogenous murine FLICE inhibitory protein (FLIP) tagged with FLAG from mouse thyroid tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with BSA prior to incubation with the antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Yujiang FANG
References for Anti-DDDDK tag antibody (ab21536)
This product has been referenced in:
- Devlin CMet al. Dihydroceramide-based Response to Hypoxia. J Biol Chem 286:38069-78 (2011). WB.Read more (PubMed: 21914808) »
- Christensen CLet al. Targeted cytosine deaminase-uracil phosphoribosyl transferase suicide gene therapy induces small cell lung cancer-specific cytotoxicity and tumor growth delay. Clin Cancer Res 16:2308-19 (2010). WB, IHC-P.Read more (PubMed: 20371678) »
See all 7 publications for this product
Publishing research using ab21536? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"