Overview

  • Product name
  • Description
    Rabbit polyclonal to DDIT3
  • Specificity
    GADD153 antibody detects endogenous levels of total GADD153 protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from human GADD153.

  • Positive control
    • LOVO cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab53081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 19 kDa).
ELISA 1/5000.

Target

  • Function
    Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
  • Involvement in disease
    Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C/EBP homologous protein antibody
    • C/EBP Homology Protein antibody
    • C/EBP zeta antibody
    • C/EBP-homologous protein 10 antibody
    • C/EBP-homologous protein antibody
    • CCAAT/enhancer binding protein homologous protein antibody
    • CEBPZ antibody
    • CHOP 10 antibody
    • CHOP antibody
    • CHOP-10 antibody
    • CHOP10 antibody
    • DDIT 3 antibody
    • DDIT-3 antibody
    • Ddit3 antibody
    • DDIT3_HUMAN antibody
    • DNA Damage Inducible Transcript 3 antibody
    • DNA damage-inducible transcript 3 protein antibody
    • GADD 153 antibody
    • GADD153 antibody
    • Growth Arrest and DNA Damage Inducible Protein 153 antibody
    • Growth arrest and DNA damage inducible protein GADD153 antibody
    • Growth arrest and DNA damage-inducible protein GADD153 antibody
    • MGC4154 antibody
    see all

Images

  • All lanes : Anti-DDIT3 antibody (ab53081) at 1/500 dilution

    Lane 1 : LOVO cell extract (treated with 10% serum for 15 mins.) with no blocking peptide
    Lane 2 : LOVO cell extract (treated with 10% serum for 15 mins.) with blocking peptide (ab53081 immunogen)


    Predicted band size : 19 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Epple LM  et al. Induction of the unfolded protein response drives enhanced metabolism and chemoresistance in glioma cells. PLoS One 8:e73267 (2013). WB . Read more (PubMed: 24039668) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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