Anti-DDIT3 (phospho S30) antibody (ab194533)
Key features and details
- Rabbit polyclonal to DDIT3 (phospho S30)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDIT3 (phospho S30) antibody
See all DDIT3 primary antibodies -
Description
Rabbit polyclonal to DDIT3 (phospho S30) -
Host species
Rabbit -
Specificity
ab194533 detects endogenous level of CHOP only when phosphorylated at serine 30. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human DDIT3 (phospho S30) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine30(V-L-S(p)-S-D) derived from Human DDIT3 .
Database link: P35638 -
Positive control
- Jurkat cell extract, treated with PMA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS (without Mg2+and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab194533 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 19 kDa. |
Target
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Function
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. -
Involvement in disease
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1649 Human
- Omim: 126337 Human
- SwissProt: P35638 Human
- Unigene: 505777 Human
- Unigene: 728989 Human
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Alternative names
- C/EBP homologous protein antibody
- C/EBP Homology Protein antibody
- C/EBP zeta antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab194533 has been referenced in 2 publications.
- Wang D et al. Quercetin ameliorates testosterone secretion disorder by inhibiting endoplasmic reticulum stress through the miR-1306-5p/HSD17B7 axis in diabetic rats. Bosn J Basic Med Sci 22:191-204 (2022). PubMed: 34582743
- Wu X et al. Mycoplasmas bovis P48 induces apoptosis in EBL cells via an endoplasmic reticulum stress-dependent signaling pathway. Vet Microbiol 255:109013 (2021). PubMed: 33676093