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Related products
- Secondary antibodies
- Lysates
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Read our guarantee »Overview
Product name
Anti-DDIT4 antibody
See all DDIT4 products (4) ...
Description
Rabbit polyclonal to DDIT4
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to 12 amino acids from near the center of human DDIT4 (NP_061931)
Positive control
Human kidney tissue lysate; Mouse kidney tissue.
Properties
Form
Liquid
Storage instructions
Store at 4°C, stable for one year. Should not be exposed to prolonged high temperatures. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> Hypoxia regulated
Cardiovascular >> Hypoxia >> Hypoxia-Regulated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab106356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.
Target
Function
Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death.
Tissue specificity
Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
Sequence similarities
Belongs to the DDIT4 family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9NX09
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DDIT4 antibody
- DDIT4_HUMAN antibody
- Dig2 antibody
see all
Database links
- Entrez Gene: 54541 Human
- Entrez Gene: 74747 Mouse
- Entrez Gene: 140942 Rat
- Omim: 607729 Human
- SwissProt: Q9NX09 Human
- SwissProt: Q9D3F7 Mouse
- SwissProt: Q8VHZ9 Rat
- Unigene: 523012 Human
see all
Anti-DDIT4 antibody images:
Western blot - DDIT4 antibody (ab106356)
Lane 1 : Anti-DDIT4 antibody (ab106356) at 0.5 µg/ml
Lane 2 : Anti-DDIT4 antibody (ab106356) at 1 µg/ml
Lane 1 : Human kidney tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDIT4 antibody (ab106356)
Immunohistochemistry detection of DDIT4 in paraffin embedded mouse kidney tissue section using ab106356 at 5 µg/ml.
References for Anti-DDIT4 antibody (ab106356)
ab106356 has not yet been referenced specifically in any publications.
Publishing research using ab106356? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"