Anti-DDX1 antibody (ab31963)

Western blot - DDX1 antibody (ab31963)

All lanes : Anti-DDX1 antibody (ab31963) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)

Lysates/proteins at 20 µg per lane.

Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H&L) at 1/15000 dilution

Performed under reducing conditions.

Predicted band size : 82 kDa
Observed band size : 82 kDa
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

Western blot - DDX1 antibody (ab31963)

All lanes : Anti-DDX1 antibody (ab31963) at 1 µg/ml

Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size : 82 kDa
Observed band size : 82 kDa

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDX1 antibody (ab31963)

Image courtesy of Human Protein Atlas. ab31963 staining DDX1 in human gall bladder. Paraffin embedded human gall bladder tissue was incubated with ab316963 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab31963 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

Immunocytochemistry/ Immunofluorescence - Anti-DDX1 antibody (ab31963)

ICC/IF image of ab31963 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31963, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.