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Western blot - DDX1 antibody (ab31963)
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Western blot - DDX1 antibody (ab31963)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDX1 antibody (ab31963)
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Product Name
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DDX1 antibody
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See all DDX1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DDX1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human DDX1.
(Peptide available as ab30615.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Cow, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate and A431 (Human epithelial carcinoma cell line) Whole Cell Lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DDX1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DDX1 antibody images, references, products related to ab31963 and other tools.
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DDX1 antibody images:
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 Western blot - DDX1 antibody (ab31963)
All lanes : DDX1 antibody (ab31963) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 82 kDa
Observed band size : 82 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDX1 antibody (ab31963)
Image courtesy of Human Protein Atlas. ab31963 staining DDX1 in human gall bladder. Paraffin embedded human gall bladder tissue was incubated with ab316963 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab31963 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
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Search PubMed (MEDLINE) for references to DDX1
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DDX1 antibody - more information
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Customer reviews (feedback) regarding DDX1 antibody |
Customer FAQs regarding DDX1 antibody |
Protocols for DDX1 antibody |
Price and availability of products related to DDX1 antibody |
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DDX1 antibody - related products:
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DDX1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DDX1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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