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Anti-DDX1 antibody (ab77213) at 5 µg/ml + Recombinant fragment of human DDX1 at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
developed using the ECL technique
Predicted band size : 82 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein
Anti-DDX1 antibody (ab77213) at 5 µg/ml + HeLa nuclear lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
developed using the ECL technique
Predicted band size : 82 kDa
Observed band size : 82 kDa
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