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Western blot - DDX1 antibody (ab77213)
(enlarge)
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Western blot - DDX1 antibody (ab77213)
(enlarge)
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Product Name
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DDX1 antibody
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See all DDX1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to DDX1
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Immunogen
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Recombinant fragment, corresponding to amino acids 642-741 of Human DDX1 with proprietary tag (NP_004930)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB, sELISA
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
sELISA: Use at an assay dependent dilution. Detection limit is approximately 10ng/ml as a capture antibody when used against the immunogen.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Hela nuclear extract
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DDX1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DDX1 antibody images, references, products related to ab77213 and other tools.
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DDX1 antibody images:
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 Western blot - DDX1 antibody (ab77213)
DDX1 antibody (ab77213) at 5 µg/ml + Recombinant fragment of human DDX1 at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
developed using the ECL technique
Predicted band size : 82 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein
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Western blot - DDX1 antibody (ab77213)
DDX1 antibody (ab77213) at 5 µg/ml + HeLa nuclear lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
developed using the ECL technique
Predicted band size : 82 kDa
Observed band size : 82 kDa
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Search PubMed (MEDLINE) for references to DDX1
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DDX1 antibody - more information
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Customer reviews (feedback) regarding DDX1 antibody |
Customer FAQs regarding DDX1 antibody |
Protocols for DDX1 antibody |
Price and availability of products related to DDX1 antibody |
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DDX1 antibody - related products:
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DDX1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DDX1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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