Anti-DDX3 antibody [2253C5a] (ab50703)
Overview
- Product nameAnti-DDX3 antibody [2253C5a]See all DDX3 primary antibodies ...
- DescriptionMouse monoclonal [2253C5a] to DDX3
- Tested applicationsWB, Dot Blot more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant fragment, corresponding to N terminal amino acids 50-200 of Human DDX3
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityProtein G purified
- Purification notesab50703 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and serile filtered.
- Clonality Monoclonal
- Clone number2253C5a
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab50703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. Predicted molecular weight: 73 kDa. |
| Dot Blot | Dot: Use at an assay dependent dilution. |
Target
- FunctionATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
- Sequence similaritiesBelongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Cellular localizationNucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
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Database links
- Entrez Gene: 1654 Human
- Omim: 300160 Human
- SwissProt: O00571 Human
- Unigene: 380774 Human
Target information above from: UniProt accession
O00571
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ATP dependent RNA helicase DDX3X antibodyATP-dependent RNA helicase DDX3X antibodyCAP Rf antibody
- DBX antibodyDDX14 antibodyDDX3X antibodyDDX3X_HUMAN antibodyDEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibodyDEAD box antibodyDEAD box protein 3 antibodyDEAD box protein 3 X-chromosomal antibodyDEAD box X isoform antibodyDEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibodyDEAD/H box 3 antibodyFibroblast Growth Factor Inducible 14 antibodyFin14 antibodyHelicase like protein 2 antibodyHelicase-like protein 2 antibodyHLP2 antibodyX isoform antibodyX-chromosomal antibody
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Anti-DDX3 antibody [2253C5a] images
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![Western blot - DDX3 antibody [2253C5a] (ab50703)](http://a.abcam.com/ps/datasheet/Images/50/ab50703/ab50703_2.jpg)
Western blot - DDX3 antibody [2253C5a] (ab50703)Anti-DDX3 antibody [2253C5a] (ab50703) at 1/100 dilution + Hela whole cell lysate at 25 µg
Predicted band size : 73 kDa
Observed band size : 73 kDa
References for Anti-DDX3 antibody [2253C5a] (ab50703)
This product has been referenced in:
- Naji S et al. Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production. Mol Cell Proteomics 11:M111.015313 (2012). WB ; Human . Read more (PubMed: 22174317) »
