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- Secondary antibodies
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Product name
Anti-DDX3 antibody
See all DDX3 products (8) ...
Description
Rabbit polyclonal to DDX3
Specificity
Detects both DDX3 and DBY
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to a 16 amino acid peptide from near the N terminus of Human DDX3
Positive control
HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Western blot - DDX3 antibody (ab37160)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ICC/IF: Use a concentration of 10 µg/ml.
Target
Function
ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
Sequence similarities
Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localization
Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
Target information above from: UniProt accessionO00571
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- X isoform antibody
- X-chromosomal antibody
- ATP dependent RNA helicase DDX3X antibody
see all
Database links
- Entrez Gene: 1654 Human
- Entrez Gene: 13205 Mouse
- Entrez Gene: 317335 Rat
- Omim: 300160 Human
- SwissProt: Q5JSI3 Human
- SwissProt: O00571 Human
- SwissProt: Q62167 Mouse
- Unigene: 380774 Human
see all
Anti-DDX3 antibody images:
Western blot - DDX3 antibody (ab37160)
Lane 1 : Anti-DDX3 antibody (ab37160) at 0.5 µg/ml
Lane 2 : Anti-DDX3 antibody (ab37160) at 1 µg/ml
Lane 1 : Human HepG2 cell lysate
Lane 2 : Human HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 73 kDa
Observed band size : 73 kDa
References for Anti-DDX3 antibody (ab37160)
This product has been referenced in:
- Oveland E et al. Ligand-induced Flt3-downregulation modulates cell death associated proteins and enhances chemosensitivity to idarubicin in THP-1 acute myeloid leukemia cells. Leuk Res 33:276-87 (2009). WB; Human.Read more (PubMed: 18691757) »
See 1 publication for this product
Publishing research using ab37160? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"