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- Secondary antibodies
- Lysates
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Product name
Anti-DDX39 antibody
See all DDX39 products (4) ...
Description
Rabbit polyclonal to DDX39
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A region within synthetic peptide: MAEQDVENDL LDYDEEEEPQ APQESTPAPP KKDIKGSYVS IHSSGFRDFL, corresponding to N terminal amino acids 1-50 of Human DDX39
MAEQDVENDLLDYDEEEEPQAPQESTPAPPKKDIKGSYVS IHSSGFRDFL
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Western blot - DDX39 antibody (ab33773)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/312500.ELISA: 1/312500.
WB: Use a concentration of 1.25 µg/ml. Detects a band of approximately 30,43 kDa (predicted molecular weight: 35,47 kDa).Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
Relevance
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.
Cellular localization
Nuclear
Alternative names
- ATP dependent RNA helicase DDX39 antibody
- BAT1 antibody
- DDXL antibody
see all
Database links
- Entrez Gene: 10212 Human
- Entrez Gene: 68278 Mouse
- Entrez Gene: 89827 Rat
- SwissProt: O00148 Human
- SwissProt: Q8VDW0 Mouse
- SwissProt: Q3UJV4 Mouse
- SwissProt: Q5U216 Rat
- Unigene: 311609 Human
see all
Anti-DDX39 antibody images:
Western blot - DDX39 antibody (ab33773)
Anti-DDX39 antibody (ab33773) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 35,47 kDa
Observed band size : 30,43 kDa (why is the actual band size different from the predicted?)
References for Anti-DDX39 antibody (ab33773)
ab33773 has not yet been referenced specifically in any publications.
Publishing research using ab33773? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"