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Product name
Anti-DDX5 antibody
See all DDX5 products (12) ...
Description
Rabbit polyclonal to DDX5
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human DDX5.
(Peptide available as ab224 12.)
Positive control
This antibody gave a positive signal in the following lysates: Hela nuclear lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Testis (Mouse) Tissue Lysate - normal tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/1000 - 1/5000. Perform heat mediated antigen retrieval via the pressure cooker method with citrate buffer pH 6.0 before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). Can be blocked with DDX5 peptide (ab22412).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
Sequence similarities
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Post-translational
modifications
Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
Cellular localization
Nucleus > nucleolus.
Target information above from: UniProt accessionP17844
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
see all
Database links
- Entrez Gene: 1655 Human
- Entrez Gene: 13207 Mouse
- Entrez Gene: 287765 Rat
- Omim: 180630 Human
- SwissProt: P17844 Human
- SwissProt: Q61656 Mouse
- Unigene: 279806 Human
- Unigene: 220038 Mouse
see all
Anti-DDX5 antibody images:
Western blot - DDX5 antibody (ab21696)
All lanes : Anti-DDX5 antibody (ab21696) at 1 µg/ml ab21696 recognizes a clear, strong band at ~ 69kDa corresponding to DDX5. A number of other, non specific bands are weakly recognized by the antibody. All bands are quenched by the addition of the blocking peptide.
Lane 1 : HeLa nuclear lysate
Lane 2 : HeLa nuclear lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size : 69 kDa
Immunocytochemistry/ Immunofluorescence - DDX5 antibody (ab21696)
ICC/IF image of ab21696 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 10 min) and incubated with the antibody (ab21696, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab21696 localises to the nucleus as expected. We believe the small amount of signal seen skirting the nucleus is due to inefficient fixation of the sample.
Western blot - DDX5 antibody (ab21696)
All lanes : Anti-DDX5 antibody (ab21696) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 69 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 170 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDX5 antibody (ab21696)
Image courtesy of Human Protein Atlas
ab21696 staining DDXA in paraffin embedded Human adrenal gland. The tissue was incubated with ab21696 (1/3000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
Further results for this antibody can be found at www.proteinatlas.org
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDX5 antibody (ab21696)
ab21696 staining mouse adrenal tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking. The primary antibody was diluted 1/1000 and incubated with the sample for 40 minutes at 20°C. A HRP conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDX5 antibody (ab21696)
ab21696 staining human anal cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in a commercially available blocking agent for 20 minutes at 20°C. The primary anibody was diluted 1/1000 and incubated with the sample for 40 minutes at 20°C. A HRP-conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
References for Anti-DDX5 antibody (ab21696)
This product has been referenced in:
- Zelenko Z et al. Nuclear receptor, coregulator signaling, and chromatin remodeling pathways suggest involvement of the epigenome in the steroid hormone response of endometrium and abnormalities in endometriosis. Reprod Sci 19:152-62 (2012). IHC-P; Human.Read more (PubMed: 22138541) »
- Laurent FX et al. New function for the RNA helicase p68/DDX5 as a modifier of MBNL1 activity on expanded CUG repeats. Nucleic Acids Res : (2011). ICC/IF; Human.Read more (PubMed: 22156369) »
See all 6 publications for this product
Publishing research using ab21696? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"