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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-DDX6 antibody
See all DDX6 products (7) ...
Description
Rabbit polyclonal to DDX6
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Chicken, Guinea pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human DDX6.
(Peptide available as ab41593.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HEK 293, A431, Jurkat.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Relevance
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
Cellular localization
Cytoplasm; P-body. Note: Processing bodies (PB).
Alternative names
- ATP dependent RNA helicase DDX6 antibody
- ATP dependent RNA helicase p54 antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
see all
Database links
- Entrez Gene: 419783 Chicken
- Entrez Gene: 1656 Human
- Entrez Gene: 13209 Mouse
- Omim: 600326 Human
- SwissProt: P26196 Human
- SwissProt: P54823 Mouse
Anti-DDX6 antibody images:
Western blot - DDX6 antibody (ab40684)
All lanes : Anti-DDX6 antibody (ab40684) at 1 µg/ml
Lane 1 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Immunocytochemistry/ Immunofluorescence - DDX6 antibody (ab40684)
ICC/IF image of ab40684 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-DDX6 antibody (ab40684)
This product has been referenced in:
- Beaudoin Set al. A large ribonucleoprotein particle induced by cytoplasmic PrP shares striking similarities with the chromatoid body, an RNA granule predicted to function in posttranscriptional gene regulation. Biochim Biophys Acta 1793:335-45 (2009). WB, ICC/IF; Mouse.Read more (PubMed: 19014979) »
See 1 publication for this product
Publishing research using ab40684? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"