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Western blot - DDX6 antibody (ab45869)
(enlarge)
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Product Name
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DDX6 antibody
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See all DDX6 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to DDX6
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Immunogen
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Synthetic peptide: C-AEYHSEPVED EKP, corresponding to C terminal amino acids 471-483 of Human DDX6
BLAST 'C-AEYHSEPVEDEKP' with  or 
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Reacts with
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Hu, Pig
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Predicted to react
(species key)
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Ms, Dog, Gpig
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate.
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Cellular localization
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Cytoplasm. Processing bodies (PB).
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DDX6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DDX6 antibody images, references, products related to ab45869 and other tools.
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DDX6 antibody images:
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 Western blot - DDX6 antibody (ab45869)
DDX6 antibody (ab45869) at 0.3 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).
Predicted band size : 54 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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References for DDX6 antibody (ab45869)
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This product has been used in:
Shen X et al. Identification of novel SHPS-1-associated proteins and their roles in regulation of insulin-like growth factor-dependent responses in vascular smooth muscle cells. Mol Cell Proteomics : (2009). WB; Pig. PubMed: 19299420
If you publish research using ab45869 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to DDX6
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DDX6 antibody - more information
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Customer reviews (feedback) regarding DDX6 antibody |
Customer FAQs regarding DDX6 antibody |
Protocols for DDX6 antibody |
Price and availability of products related to DDX6 antibody |
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DDX6 antibody - related products:
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DDX6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DDX6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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