Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-DDX6 antibody
See all DDX6 products (7) ...
Description
Goat polyclonal to DDX6
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human, Pig
Predicted to work with
Mouse, Guinea pig, Dog
Immunogen
Synthetic peptide: C-AEYHSEPVED EKP, corresponding to C terminal amino acids 471-483 of Human DDX6
C-AEYHSEPV EDEKP
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
-
Western blot - DDX6 antibody (ab45869)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
Peptide ELISA: Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
Cellular localization
Cytoplasm; P-body. Note: Processing bodies (PB).
Alternative names
- ATP dependent RNA helicase DDX6 antibody
- ATP dependent RNA helicase p54 antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
see all
Database links
- Entrez Gene: 1656 Human
- Entrez Gene: 13209 Mouse
- Omim: 600326 Human
- SwissProt: P26196 Human
- SwissProt: P54823 Mouse
Anti-DDX6 antibody images:
Western blot - DDX6 antibody (ab45869)
Anti-DDX6 antibody (ab45869) at 0.3 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).
Predicted band size : 54 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-DDX6 antibody (ab45869)
This product has been referenced in:
- Shen Xet al. Identification of novel SHPS-1-associated proteins and their roles in regulation of insulin-like growth factor-dependent responses in vascular smooth muscle cells. Mol Cell Proteomics : (2009). WB; Pig.Read more (PubMed: 19299420) »
See 1 publication for this product
Publishing research using ab45869? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"