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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-DDX6 antibody
See all DDX6 products (7) ...
Description
Rabbit polyclonal to DDX6
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a sequence between N terminal residues 1 to 50 of human DDX6 (Swiss-Prot P26196).
Positive control
Whole cell lysate from HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab70455 was affinity purified using an epitope specific to DDX6 immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/15000.Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Relevance
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
Cellular localization
Cytoplasm; P-body. Note: Processing bodies (PB).
Alternative names
- ATP dependent RNA helicase DDX6 antibody
- ATP dependent RNA helicase p54 antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
see all
Database links
- Entrez Gene: 1656 Human
- Entrez Gene: 13209 Mouse
- Omim: 600326 Human
- SwissProt: P26196 Human
- SwissProt: P54823 Mouse
Anti-DDX6 antibody images:
Western blot - DDX6 antibody (ab70455)
All lanes : Anti-DDX6 antibody (ab70455) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Predicted band size : 54 kDa
Observed band size : 54 kDa
Immunoprecipitation - DDX6 antibody (ab70455)
250µg HeLa whole cell lysate treated with ab70455 at 0.04 µg/ml for the Western blot, and at 3 µg/mg lysate for Immunoprecipitation.
References for Anti-DDX6 antibody (ab70455)
ab70455 has not yet been referenced specifically in any publications.
Publishing research using ab70455? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"