Overview

  • Product nameAnti-DEBS2 antibody
    See all DEBS2 primary antibodies
  • Description
    Mouse polyclonal to DEBS2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with Saccharopolyspora erythraea.
  • Immunogen

    Recombinant fusion protein: QPLRAGEVRV DVRATGVNFR DVLLALGMYP QKADMGTEAA GVVTAVGPDV DAFAPGDRVL GLFQGAFAPI AVTDHRLLAR VPDGWSDADA AAVPIAYTTA, corresponding to amino acids 2846-2945 of DEBS2 (Saccharopolyspora erythraea)

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab52733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Detects a band of approximately 25 kDa.

    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDEBS2 is a protein involved in polyketide biosynthesis. Polyketides are secondary metabolites. They are a structurally diverse family of natural products with a broad range of biological activities. The formation of polyketides is very similar to the biosynthesis of long chain fatty acids – both in the enzymatic reactions that take place and the enzyme proteins that are involved. During the last decade many polyketide gene-clusters have been cloned and sequenced. DNA sequencing has shown that the clusters have substantial homology suggesting that they originated from a common ancestor. The type I PKS gene-clusters, such as those responsible for the biosynthesis of complex polyketides like the macrolide erythromycin, are multi-functional enzymes with a modular organisation. Each module is responsible for a single cycle of polyketide carbon chain extension and contains domains for necessary reduction activities. Therefore, there is a one-to-one correspondence between the product structure and the active domains in modular PKSs. DEBS1, DEBS2 and DEBS3 are modular proteins, their corresponding genes being eryAI, eryAII and eryAIII. These genes encode the type I polyketide synthase complex responsible for the production of erythromycin A.
    • Alternative names
      • 6-deoxyerythronolide B synthase II antibody
      • EryAII antibody

    Anti-DEBS2 antibody images

    • All lanes : Anti-DEBS2 antibody (ab52733) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen (tagged recombinant fusion protein)

      Secondary
      Lane 1 : Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution
      Lane 2 : Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution


      Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    References for Anti-DEBS2 antibody (ab52733)

    ab52733 has not yet been referenced specifically in any publications.

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