![Immunoprecipitation - Anti-DECR1 antibody [8B1AD10] (ab110287) image](#DECR1-Primary-antibodies-ab110287-8.gif)
![Flow Cytometry - DECR1 antibody [8B1AD10] (ab110287) image](#DECR1-Primary-antibodies-ab110287-5.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DECR1 antibody [8B1AD10] (ab110287) image](#DECR1-Primary-antibodies-ab110287-7.gif)
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Product name
Anti-DECR1 antibody [8B1AD10]
See all DECR1 products (4) ...
Description
Mouse monoclonal [8B1AD10] to DECR1
Tested applications
IP, ICC/IF, IHC-P, In-Cell ELISA, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Human liver mitochondria.
Positive control
MRC5 fibroblasts; Human cerebellum tissue; Human liver mitochondria; HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
8B1AD10
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
IP: Use at an assay dependent dilution. This antibody will only immunoprecipitate DECR1 from isolated mitochondria.
ICC/IF: Use a concentration of 1 µg/ml.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
In-Cell ELISA: Use a concentration of 4 µg/ml. (0.4 µg/well)
Flow Cyt: Use a concentration of 1 µg/ml.
Target
Function
Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.
Tissue specificity
Heart = liver = pancreas > kidney >> skeletal muscle = lung.
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionQ16698
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2,4 dienoyl CoA reductase [NADPH] antibody
- Nadph antibody
- RP23-338J24.3 antibody
see all
Database links
- Entrez Gene: 1666 Human
- Omim: 222745 Human
- SwissProt: Q16698 Human
- Unigene: 492212 Human
Anti-DECR1 antibody [8B1AD10] images:
Immunoprecipitation - Anti-DECR1 antibody [8B1AD10] (ab110287)
![Immunoprecipitation - Anti-DECR1 antibody [8B1AD10] (ab110287)](/ps/datasheet/images/110/ab110287/DECR1-Primary-antibodies-ab110287-8.gif)
DECR1 immunocaptured from human liver mitochondria using ab110287.
Flow Cytometry - DECR1 antibody [8B1AD10] (ab110287)
![Flow Cytometry - DECR1 antibody [8B1AD10] (ab110287)](/ps/datasheet/images/110/ab110287/DECR1-Primary-antibodies-ab110287-5.jpg)
HeLa cells were stained with 1 µg/mL ab110287 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DECR1 antibody [8B1AD10] (ab110287)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DECR1 antibody [8B1AD10] (ab110287)](/ps/datasheet/images/110/ab110287/DECR1-Primary-antibodies-ab110287-7.gif)
ab110287 staining DECR1 in formalin-fixed, paraffin-embedded Human cerebellum tissue by Immunohistochemistry. DECR1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
Immunocytochemistry/ Immunofluorescence - Anti-DECR1 antibody [8B1AD10] (ab110287)
![Immunocytochemistry/ Immunofluorescence - Anti-DECR1 antibody [8B1AD10] (ab110287)](/ps/datasheet/images/110/ab110287/DECR1-Primary-antibodies-ab110287-9.gif)
MRC5 fibroblasts labeled with 1 µg/ml ab110287 and detected with goat anti-mouse secondary conjugated to FITC.
References for Anti-DECR1 antibody [8B1AD10] (ab110287)
ab110287 has not yet been referenced specifically in any publications.
Publishing research using ab110287? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"