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Products:Cell Biology >> Apoptosis >> Nucleus >> DNAses

Anti-DFFB antibody [36A349] (ab13592)

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Overview

Product name

Anti-DFFB antibody [36A349]
See all DFFB products (12) ...

Description

Mouse monoclonal [36A349] to DFFB

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide: SYFDRGAKGGSRLCTPEG, corresponding to amino acids 205 - 222 of Human DFFB

SYFDRGAKGG SRLCTPEG

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

36A349

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Cell Biology >> Apoptosis >> Nucleus >> Chromatin Condensation
Cell Biology >> Apoptosis >> Nucleus >> DNAses

  • Western blot - DFFB antibody [36A349] (ab13592)Western blot - DFFB antibody [36A349] (ab13592) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 2 µg/mlDetects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).

Target

Relevance

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. The DNA fragmentation factor (DFF) described by Liu et al. (1997) is a heterodimer of 40 kD (DFFB) and 45 kD (DFFA; 601882) subunits.

Cellular localization

Cytoplasmic and Nuclear

Alternative names

  • 5730477D02Rik antibody
  • CAD antibody
  • Caldesmon antibody
  • Caspase activated deoxyribonuclease antibody
  • Caspase activated DNase antibody
  • Caspase activated nuclease antibody
  • CDM antibody
  • CPAN antibody
  • Deoxyribonuclease III, caspase activated antibody
  • DFF 40 antibody
  • DFF2 antibody
  • DFF40 antibody
  • Didff antibody
  • DNA fragmentation factor 40 kDa subunit antibody
  • DNA fragmentation factor subunit beta antibody
  • DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody
  • DNA fragmentation factor, 40 kD beta subunit antibody
  • DNA fragmentation factor, 40 kD, beta polypeptide antibody
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) antibody
  • DNAation factor, beta subunit antibody
  • OTTHUMP00000003633 antibody
see all

Database links

Anti-DFFB antibody [36A349] images:

  Western blot - DFFB antibody [36A349] (ab13592)

Western blot - DFFB antibody [36A349] (ab13592)

Anti-DFFB antibody [36A349] (ab13592) at 2 µg/ml + 30ug HeLa cell lysate

Predicted band size : 41 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-DFFB antibody [36A349] (ab13592)

ab13592 has not yet been referenced specifically in any publications.

Publishing research using ab13592? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"