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Western blot - CAD antibody (ab8400)
Western blot - CAD antibody (ab8400)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAD antibody (ab8400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAD antibody (ab8400)
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Product Name 

DFFB antibody

 

See all DFFB antibodies (12)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to DFFB

Immunogen 

Synthetic peptide, corresponding to C terminal amino acids 314-329 of Mouse DFFB(Peptide available as ab8432.)

Reacts with 
(species key)

Ms

Tested applications 
(see key)

IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml.
WB: 1/500 - 1/1000. Predicted molecular weight: 39 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tissue lysate from murine lung or kidney.
A 40 kDa band should be detected.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Cell Biology >> Apoptosis >> Nucleus >> Chromatin Condensation
Cell Biology >> Apoptosis >> Nucleus >> DNAses

Relevance 

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. The DNA fragmentation factor (DFF) described by Liu et al. (1997) is a heterodimer of 40 kD (DFFB) and 45 kD (DFFA; 601882) subunits.

 

DFFB antibody (ab8400)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

13368    (Mouse)

SwissProt

    

O54788    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DFFB antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for DFFB antibody images, references, products related to ab8400 and other tools.

 

DFFB antibody images:

Product image  Western blot - CAD antibody (ab8400)

Western blot - CAD antibody (ab8400)

All lanes : DFFB antibody (ab8400) at 1/500 dilution

Lane 1 : Murine lung tissue lysate
Lane 2 : Murine kidney tissue lysate


Predicted band size : 39 kDa

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAD antibody (ab8400)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAD antibody (ab8400)

ab8400 at 1µg/ml staining CAD in mouse kidney tissue by IHC

 
 

Search PubMed (MEDLINE) for references to DFFB

 

DFFB antibody - more information

Customer reviews (feedback) regarding DFFB antibody

Customer FAQs regarding DFFB antibody

Protocols for DFFB antibody

Price and availability of products related to DFFB antibody

 

DFFB antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for DFFB antibody

ab8432

  

CAD (C-term) peptide (mouse)

 

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Other DFFB Antibodies

See all DFFB antibodies (12)...

 

DFFB antibody - other tools:

Contact Abcam with a Technical Enquiry about DFFB antibody

 

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