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Products:Cell Biology >> Apoptosis >> Nucleus >> DNAses
Overview
Product name
Anti-DFFB antibody
See all DFFB products (12) ...
Description
Rabbit polyclonal to DFFB
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: QKPKSVKLRALRSPRKC, corresponding to amino acids 3/18 of Human DFFB (Peptide available as ab8440.)
QKPKSVKLRA LRSPRKC
Positive control
K562 or Jurkat whole cell lysate A 40 kDa band can be detected.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Cell Biology >> Apoptosis >> Nucleus >> Chromatin Condensation
Cell Biology >> Apoptosis >> Nucleus >> DNAses
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: 1/500 - 1/2000.Detects a band of approximately 40 kDa.Can be blocked with DFFB peptide (3/18) (ab8440).
Target
Relevance
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. The DNA fragmentation factor (DFF) described by Liu et al. (1997) is a heterodimer of 40 kD (DFFB) and 45 kD (DFFA; 601882) subunits.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- 5730477D02Rik antibody
- CAD antibody
- Caldesmon antibody
see all
Database links
- Entrez Gene: 1677 Human
- Omim: 601883 Human
- SwissProt: O76075 Human
Anti-DFFB antibody images:
Western blot - DFFB antibody (ab8408)
All lanes : Anti-DFFB antibody (ab8408) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : K562 whole cell lysate
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - DFFB antibody (ab8408)
ab8408 at 5µg/ml staining DFFBin Jurkat cells by ICC/IF
References for Anti-DFFB antibody (ab8408)
ab8408 has not yet been referenced specifically in any publications.
Publishing research using ab8408? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"