Overview

  • Product name
  • Description
    Rabbit polyclonal to DGAT2L4
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C-terminal sequence amino acids 284-333 (GEPLPMPKIE NPSQEIVAKY HTLYIDALRK LFDQHKTKFG ISETQELEII) of human DGAT2L4 (NP_001002254).

  • Positive control
    • Fetal brain lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83182 is purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:12500.

Target

  • Function
    Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has no activity using decyl alcohol and significantly prefers the C16 and C18 alcohols. May also have 2-acylglycerol O-acyltransferase (MGAT) and acyl-CoA:retinol acyltransferase (ARAT) activities, to catalyze the synthesis of diacylglycerols and retinyl esters; however this activity is unclear in vivo.
  • Tissue specificity
    Abundant in tissues rich in sebaceous glands such as the preputial gland and eyelid. Highly expressed in skin, where it is primarily restricted to undifferentiated peripheral sebocytes. Also expressed at lower level in other tissues except pancreas.
  • Sequence similarities
    Belongs to the diacylglycerol acyltransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acyl CoA wax alcohol acyltransferase 2 antibody
    • Acyl-CoA retinol O-fatty-acyltransferase antibody
    • Acyl-CoA wax alcohol acyltransferase 2 antibody
    • ARAT antibody
    • Awat2 antibody
    • AWAT2_HUMAN antibody
    • DC4 antibody
    • Diacylglycerol O acyltransferase 2 like 4 antibody
    • Diacylglycerol O acyltransferase candidate 4 antibody
    • Diacylglycerol O-acyltransferase 2-like protein 4 antibody
    • Diacylglycerol O-acyltransferase candidate 4 antibody
    • hDC4 antibody
    • hWS antibody
    • Long chain alcohol O fatty acyltransferase 2 antibody
    • Long-chain-alcohol O-fatty-acyltransferase 2 antibody
    • MFAT antibody
    • Multifunctional O acyltransferase antibody
    • Multifunctional O-acyltransferase antibody
    • Retinol O-fatty-acyltransferase antibody
    • Wax synthase 2 antibody
    • Wax synthase antibody
    • WS antibody
    see all

Anti-DGAT2L4 antibody images

  • Anti-DGAT2L4 antibody (ab83182) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

References for Anti-DGAT2L4 antibody (ab83182)

This product has been referenced in:
  • Kaylor JJ  et al. Identification of the 11-cis-specific retinyl-ester synthase in retinal Müller cells as multifunctional O-acyltransferase (MFAT). Proc Natl Acad Sci U S A 111:7302-7 (2014). Read more (PubMed: 24799687) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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