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Overview

  • Product nameAnti-DGCR8 antibodySee all DGCR8 primary antibodies ...
  • Description
    Rabbit polyclonal to DGCR8
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 180-229 (DKKDEENELD QEKRVEYAVL DELEDFTDNL ELDEEGAGGF TAKAIVQRDR) of human DGCR8 (NP_073557).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHela cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    ELISA titre using peptide based assay, 1:312500
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionComponent of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.
    • Tissue specificityUbiquitously expressed.
    • Sequence similaritiesContains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 WW domain.
    • DomainBoth DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.
    • Cellular localizationNucleus. Nucleus > nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.

      • Target information above from: UniProt accession Q8WYQ5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        DGCRK6 antibodyC22orf12 antibodyD16H22S788E antibody
        D16Wis2 antibodyDGCR 8 antibodyDgcr8 antibodyDGCR8_HUMAN antibodyDGCRK 6 antibodyDiGeorge syndrome critical region 8 antibodyDiGeorge syndrome critical region gene 8 antibodyGy1 antibodyMicroprocessor complex subunit DGCR8 antibodypasha antibody
      see all

    Anti-DGCR8 antibody images

    • Western blot - DGCR8 antibody (ab82876)
      Western blot - DGCR8 antibody (ab82876)
      Anti-DGCR8 antibody (ab82876) at 1 µg/ml + Hela cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 86 kDa
      Observed band size : 86 kDa
      Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-DGCR8 antibody(ab82876)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-DGCR8 antibody(ab82876)
      Immunohistochemistry of Liver tissue at an antibody concentration of 4-8µg/ml using anti-DGCR8 antibody (ab82876)

    References for Anti-DGCR8 antibody (ab82876)

    This product has been referenced in:

    See all 2 Publications for this product

    Publishing research using ab82876 ? Please let us know so that we can cite the reference in this datasheet

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