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Synthetic peptide corresponding to a region within N terminal amino acids 180-229 (DKKDEENELD QEKRVEYAVL DELEDFTDNL ELDEEGAGGF TAKAIVQRDR) of human DGCR8 (NP_073557).
Our Abpromise guarantee covers the use of ab82876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:312500.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) ananlysis of human liver tissue labelling DGCR8 with ab82876 at 4-8µg/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"