Overview

  • Product nameAnti-DHX30 antibodySee all DHX30 primary antibodies ...
  • Description
    Rabbit polyclonal to DHX30
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab59126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

Anti-DHX30 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adult liver labelling DHX30 with ab59126 at 1/100. A Cy3 conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Magnification: 20X. Staining is seen in the cytoplasm of hepatocytes.

    Left - DAPI, Middle - DHX30, Right - Merge.

  • Anti-DHX30 antibody (ab59126) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa

References for Anti-DHX30 antibody (ab59126)

ab59126 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and your interest in our products. The exact immunogen sequence could hit Q7L2E3-2 only according to the sequence alignment, thus we think the antibody could only recognize isoform 2 of DHX30 protein. I hope this inf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"