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Synthetic peptide (Human)
Our Abpromise guarantee covers the use of ab59126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adult liver labelling DHX30 with ab59126 at 1/100. A Cy3 conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Magnification: 20X. Staining is seen in the cytoplasm of hepatocytes.
Left - DAPI, Middle - DHX30, Right - Merge.
ab59126 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"