Anti-DHX30 antibody (ab85754)
Key features and details
- Rabbit polyclonal to DHX30
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHX30 antibody
See all DHX30 primary antibodies -
Description
Rabbit polyclonal to DHX30 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region between residues 1144 and 1194 of human DHX30 (NP_619520.1)
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85754 was affinity purified using an epitope specific to DHX30 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
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IP |
Use at 5-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). |
IP
Use at 5-10 µg/mg of lysate. |
Target
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Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsIsoform 2 is phosphorylated on Ser-15. -
Cellular localization
Cytoplasm and Mitochondrion. Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid. - Information by UniProt
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Database links
- Entrez Gene: 22907 Human
- Entrez Gene: 367172 Rat
- GenBank: NM_138614 Human
- SwissProt: Q7L2E3 Human
- SwissProt: Q7L2E3-2 Human
- SwissProt: Q5BJS0 Rat
- Unigene: 517948 Human
- Unigene: 163034 Rat
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Alternative names
- DDX30 antibody
- DEAH box protein 30 antibody
- Dhx30 antibody
see all
Images
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All lanes : Anti-DHX30 antibody (ab85754) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 110 kDa, 180 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of Human DHX3 by Western Blot of Immunprecipitate.
All lanes: ab85754 at 0.4µg/ml staining DHX3 in HeLa whole cell lysates immunoprecipitated at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
Lane 1: ab85754
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85754 has not yet been referenced specifically in any publications.