Overview

  • Product name
  • Description
    Rabbit polyclonal to DHX57
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 150 and 200 of human DHX57 (NP_945314.1).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab86784 was affinity purified using an epitope specific to DHX57 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 156 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probable ATP-binding RNA helicase.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DEAH subfamily.
      Contains 1 C3H1-type zinc finger.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 UBA domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • DDX57 antibody
      • DEAH (Asp Glu Ala Asp/His) box polypeptide 57 antibody
      • DEAH box protein 57 antibody
      • DEAH box RNA/DNA helicase AAM73547 antibody
      • DHX 57 antibody
      • DHX57 antibody
      • DHX57_HUMAN antibody
      • FLJ32861 antibody
      • Putative ATP dependent RNA helicase DHX57 antibody
      • Putative ATP-dependent RNA helicase DHX57 antibody
      see all

    Images

    • All lanes : Anti-DHX57 antibody (ab86784) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 156 kDa
      Observed band size : 171 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of DHX57 by Western Blot of Immunprecipitate.
      ab86784, at 0.4 µg/ml, staining DHX57 in HeLa whole cell lysate immunoprecipitated using ab86784 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab86784 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab86784.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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