Overview

  • Product nameAnti-DHX8 antibody
    See all DHX8 primary antibodies
  • Description
    Mouse monoclonal to DHX8
  • Tested applicationsWB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: RREGRVANVA DVVSKGQRVK VKVLSFTGTK TSLSMKDVDQ ETGEDLNPNR RRNLVGETNE ETSMRNPDRP THLSLVSAPE VEDDSLERKR LTRISDPEKW , corresponding to amino acids 301-401 of Human DHX8

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab54592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells.

Target

  • FunctionFacilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 S1 motif domain.
  • DomainThe RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DHX8 antibody
    • ATP-dependent RNA helicase dhx8 antibody
    • DDX 8 antibody
    • DDX8 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 (RNA helicase) antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 antibody
    • DEAD/H box polypeptide 8 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 8 antibody
    • DEAH box polypeptide 8 antibody
    • DEAH box protein 8 antibody
    • DHX 8 antibody
    • dhx8 antibody
    • DHX8_HUMAN antibody
    • HRH 1 antibody
    • HRH1 antibody
    • PRP 22 antibody
    • PRP22 antibody
    • PRPF 22 antibody
    • PRPF22 antibody
    • RNA helicase HRH 1 antibody
    • RNA helicase HRH1 antibody
    see all

Anti-DHX8 antibody images

  • DHX8 antibody (ab54592) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab54592 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54592, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-DHX8 antibody (ab54592)

ab54592 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"