Anti-DHX8 antibody (ab54592)

Overview

• Product nameAnti-DHX8 antibodySee all DHX8 primary antibodies ...
• Description
  Mouse monoclonal to DHX8
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment: RREGRVANVA DVVSKGQRVK VKVLSFTGTK TSLSMKDVDQ ETGEDLNPNR RRNLVGETNE ETSMRNPDRP THLSLVSAPE VEDDSLERKR LTRISDPEKW , corresponding to amino acids 301-401 of Human DHX8

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Splicing

Applications

Target

• FunctionFacilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
• Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
  Contains 1 helicase ATP-binding domain.
  Contains 1 helicase C-terminal domain.
  Contains 1 S1 motif domain.
• DomainThe RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q14562 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1659 Human
  • Omim: 600396 Human
  • SwissProt: Q14562 Human
  • Unigene: 463105 Human

• Alternative names
  ATP dependent RNA helicase DHX8 antibodyATP-dependent RNA helicase dhx8 antibodyDDX 8 antibody

  DDX8 antibodyDEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 (RNA helicase) antibodyDEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 antibodyDEAD/H box polypeptide 8 antibodyDEAH (Asp Glu Ala His) box polypeptide 8 antibodyDEAH box polypeptide 8 antibodyDEAH box protein 8 antibodyDHX 8 antibodydhx8 antibodyDHX8_HUMAN antibodyHRH 1 antibodyHRH1 antibodyPRP 22 antibodyPRP22 antibodyPRPF 22 antibodyPRPF22 antibodyRNA helicase HRH 1 antibodyRNA helicase HRH1 antibody

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Anti-DHX8 antibody images

• 

  Western blot - DHX8 antibody (ab54592)
  DHX8 antibody (ab54592) at 1ug/lane + HeLa cell lysate at 25ug/lane.
• 

  Flow Cytometry-Anti-DHX8 antibody(ab54592)
  Overlay histogram showing HeLa cells stained with ab54592 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54592, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.