Anti-DIS antibody (ab70243)
Key features and details
- Rabbit polyclonal to DIS
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DIS antibody
See all DIS primary antibodies -
Description
Rabbit polyclonal to DIS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human DIS. (SwissProt Q8IX12)
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Positive control
- Nuclear extract from HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
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IP |
Use at 1-10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). |
IP
Use at 1-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation. -
Tissue specificity
Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors. -
Sequence similarities
Contains 1 SAP domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 423692 Chicken
- Entrez Gene: 450498 Chimpanzee
- Entrez Gene: 767587 Cow
- Entrez Gene: 479230 Dog
- Entrez Gene: 55749 Human
- Entrez Gene: 67500 Mouse
- Entrez Gene: 361849 Rat
- Omim: 612569 Human
see all -
Alternative names
- CARP-1 antibody
- Carp1 antibody
- Ccar1 antibody
see all
Images
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All lanes : Anti-DIS antibody (ab70243) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 132 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DIS with ab70243 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells (lanes 1 and 2). ab70243 was used for IP at 6 µg/mg lysate. For western blot ab70243 was used at 1 µg/ml. Lane 3 - control IgG.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab70243 has been referenced in 2 publications.
- Fei X et al. TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-I. Mol Ther Oncolytics 26:413-428 (2022). PubMed: 36159777
- Li D et al. RP11-284F21.9 promotes lung carcinoma proliferation and invasion via the regulation of miR-627-3p/CCAR1. Oncol Rep 44:1638-1648 (2020). PubMed: 32945522