Overview

  • Product nameAnti-DIS3 antibodySee all DIS3 primary antibodies ...
  • Description
    Mouse polyclonal to DIS3
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length DIS3 protein (Human)

  • Positive control
    • KIAA1008 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 109 kDa).
ICC/IF Use at an assay dependent dilution. PubMed: 21078874

Target

  • FunctionPutative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the RNR ribonuclease family.
    Contains 1 PINc domain.
  • Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810028N01Rik antibody
    • bA555G22 antibody
    • Dis3 antibody
    • DIS3 mitotic control homolog (S. cerevisiae) antibody
    • DIS3 mitotic control, S. cerevisiae, homolog of antibody
    • dis3p antibody
    • EXOSC11 antibody
    • Exosome complex exonuclease RRP44 antibody
    • exosome component 11 antibody
    • KIAA1008 antibody
    • Mitotic control protein dis3 homolog antibody
    • Protein DIS3 homolog antibody
    • Ribosomal RNA processing protein 44 antibody
    • Ribosomal RNA processing protein 44, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 44 antibody
    • RP11-342J4.3 antibody
    • RRP44 antibody
    • RRP44_HUMAN antibody
    see all

Anti-DIS3 antibody images

  • All lanes : Anti-DIS3 antibody (ab68570) at 1/500 dilution

    Lane 1 : DIS3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 109 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References for Anti-DIS3 antibody (ab68570)

This product has been referenced in:
  • Pfeiffer JR  et al. CARHSP1 is required for effective tumor necrosis factor alpha mRNA stabilization and localizes to processing bodies and exosomes. Mol Cell Biol 31:277-86 (2011). ICC/IF ; Mouse . Read more (PubMed: 21078874) »
  • Ayala YM  et al. TDP-43 regulates its mRNA levels through a negative feedback loop. EMBO J 30:277-88 (2011). WB ; Human . Read more (PubMed: 21131904) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

I can confirm that this antibody has been tested on endogenous samples in WB and ICC-IF in the two publications provided on the datasheet:

Pfeiffer JR et al. CARHSP1 is required for effective tumor necr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"