Anti-DIS3 antibody (ab68570)
- Product nameAnti-DIS3 antibodySee all DIS3 primary antibodies ...
- DescriptionMouse polyclonal to DIS3
- SpecificityThis antibody is reactive against mammalian transfected lysate.
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Full length DIS3 protein (Human)
- Positive control
- KIAA1008 transfected 293T cell lysate
- Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.2
- Concentration information loading...
- PurityProtein A purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab68570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 109 kDa).|
|ICC/IF||ICC/IF: Use at an assay dependent dilution. PubMed: 21078874|
- FunctionPutative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
- Tissue specificityWidely expressed.
- Sequence similaritiesBelongs to the RNR ribonuclease family.
Contains 1 PINc domain.
- Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3.
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Anti-DIS3 antibody images
All lanes : Anti-DIS3 antibody (ab68570) at 1/500 dilution
Lane 1 : DIS3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 109 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
References for Anti-DIS3 antibody (ab68570)
This product has been referenced in:
- Pfeiffer JR et al. CARHSP1 is required for effective tumor necrosis factor alpha mRNA stabilization and localizes to processing bodies and exosomes. Mol Cell Biol 31:277-86 (2011). ICC/IF ; Mouse . Read more (PubMed: 21078874) »
- Ayala YM et al. TDP-43 regulates its mRNA levels through a negative feedback loop. EMBO J 30:277-88 (2011). WB ; Human . Read more (PubMed: 21131904) »