Mouse DKK1 ELISA Kit (Dickkopf-1) (ab113347)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse DKK1 ELISA Kit (Dickkopf-1)
See all DKK1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 25 pg/ml -
Range
8.23 pg/ml - 6000 pg/ml -
Recovery
90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 79.55 71% - 85% Plasma 91.38 83% - 100% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s DKK1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DKK1 in plasma and cell culture supernatants. (Mouse DKK1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse DKK1 in normal serum with ab113347, only in serum spiked with Mouse DKK1.
Get better reproducibility in only 90 minutes with Mouse DKK1 ELISA Kit (ab197746) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse DKK1 2 vials DKK1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse DKK1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
DKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. -
Alternative names
- Dickkopf 1
- Dickkopf homolog 1
- Dickkopf related protein 1
see all -
Database links
- Entrez Gene: 13380 Mouse
- SwissProt: O54908 Mouse
Associated products
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SimpleStep ELISA kits
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab113347 has been referenced in 1 publication.
- Li Y et al. Experimental study on the effects of simvastatin in reversing the femoral metaphyseal defects induced by sodium valproate in normal and ovariectomized rats. Heliyon 8:e10480 (2022). PubMed: 36105473