Overview
- Product nameDKK1 Human ELISA KitSee all DKK1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 100 pg/ml
- Range122.9 pg/ml - 30000 pg/ml
- Recovery
90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 108.3 93% - 123% Serum 92.3 62% - 130% Plasma 72.8 59% - 110% - Species reactivityReacts with: Human
- Product overview
ab100501 DKK1 (Dickkopf homolog 1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DKK1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Notes
Optimization may be required with urine samples.
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 700X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/ cell culture medium/urine) diluent. 1 x 15ml Detection Antibody Dkk-1: biotinylated anti-human Dkk-1 2 vials DKK 1 Standard Human (recombinant) 2 vials Dkk1 Microplate (12 strips x 8 wells) coated with anti-human Dkk-1 1 unit Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml -
Research Areas
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Alternative names
- Dickkopf 1Dickkopf homolog 1 Dickkopf related protein 1
- DKK 1hDkk 1 hDkk1 mDkk1SK
see all
- Entrez Gene: 22943 Human
- Omim: 605189 Human
- SwissProt: O94907 Human
Applications
Our Abpromise guarantee covers the use of ab100501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
DKK1 Human ELISA Kit images
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ELISA - DKK1 Human ELISA Kit (ab100501)
Protocols
References for DKK1 Human ELISA Kit (ab100501)
ab100501 has not yet been referenced specifically in any publications.
